Clustered Regularly Interspaced Short Palindromic Repeat Paired Associated Protein 9 (CRISPR-Cas9) System and Its Opportunity in Medical Science - A Narrative Review

The clustered regularly interspaced short palindromic repeat paired associated protein 9 (CRISPR-Cas9) is a site-specific genome editing tool that enables scientists to edit or introduce genetic mutation at will. CRISPR-Cas9 consists of two essential key players; programmable RNA called single guide (sgRNA) and the Cas9 which functions as molecular scissors does cutting. Since its discovery, has received vast attention due simplicity, convenience, superior precision use. Its application extends into various fields including health sciences where it been used enhance understanding pathogenesis help in therapeutic intervention. Despite promising potentials applications CRISPR-Cas9, there are several aspects need be addressed method delivery, off-target cutting ethical issues human germline modification. purposes this review perform comprehensive literature search publications on system highlight potential field medical sciences. In present review, we discuss background mechanisms modification use study animal model production, genetics, multifactorial complex diseases. addition, also limitations with application. accelerated studies facillitate collection amounts information. However, should further studied order reap greatest benefits.